human high sensitivity cytokine base kit a  (R&D Systems)

Journal: Preventive Nutrition and Food Science

Article Title: Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions

doi: 10.3746/pnf.2025.30.4.312

Figure Lengend Snippet: Effects of metformin on cytokine expression and ERK signaling in RPMI 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.

Article Snippet: The supernatant from RPMI 2650 cancer cells was used in the human cytokine array kit (R&D Systems) following the manufacturer’s instructions.

Techniques: Expressing, Standard Deviation

Journal: iScience

Article Title: Estrogen-mediated corneal collagen degradation in keratoconus

doi: 10.1016/j.isci.2025.113004

Figure Lengend Snippet: Sex-specific changes in tears cytokine profiles and the effect of KC and non-KC tears treatment on MMP2/9 and PLA2G2A axis in co-culture model (A) Cytokine array using proteome profiles of KC and non- KC patients (male vs. female) ( N = 3/group). (B) Principal component analysis of KC and non- KC patients (male and female) ( N = 3/group). (C and D) Pathway analysis cellular component and molecular function of KC and non- KC patients (male and female). (E) Pictorial representation of KC and non-KC patients tear fluids and β-estradiol treatment on the co-culture model apically. (F) Zymography of apical and basal conditioned medium collected from 7 days co-culture ( N = 3–4 biological replicates) ∗ p < 0.05; ∗∗ p < 0.01 Student’s t test. (G and H) Zymography and immunoblotting of PLA2G2A of apical conditioned medium after treating with KC and non -KC tear fluids apically. ( N = 3 each group), ∗ p < 0.05; ∗∗ p < 0.01 Student’s t test. Data are represented as mean ± SEM. All raw gel blots normalized using SYPRO total protein stain (See A–S4D).

Article Snippet: Cytokine detection in the tear fluid of KC and non-KC patients, as well as in conditioned medium, was conducted using array-based technology Proteome Profiler Human XL Cytokine Array Kit (Cat no ARY022B, R and D Systems, Minneapolis, USA) following the manufacturer’s instructions.

Techniques: Co-Culture Assay, Zymography, Western Blot, Staining

Journal: iScience

Article Title: Estrogen-mediated corneal collagen degradation in keratoconus

doi: 10.1016/j.isci.2025.113004

Figure Lengend Snippet: SB202190 regulates β-estradiol mediated collagen fiber degradation in corneal epithelium fibroblast 3D collagen hydrogel model (A) Pictorial representation TEM of 3D hydrogel with corneal epithelium, fibroblast, and collagen fibers. (B) TEM showing collagen fiber degradation and its quantification after treatment with β-estradiol (N = 3–4 biological replicates), ∗ p < 0.05; ∗∗ p < 0.1 Student’s t test Scale bar: 500 nm. (C) Cytokine array showing the effect of β-estradiol alone and β-estradiol followed by SB202190 treatment in 3D hydrogel model ( N = 3). (D) Zymography of conditioned medium of 3D collagen hydrogel model after treatment of β-estradiol alone and β-estradiol followed by SB202190 treatment. ( N = 3–4 biological replicates), ∗ p < 0.05; ∗∗ p < 0.01 one-way ANOVA All raw gel blots normalized using SYPRO total protein stain (See A and S7B).

Article Snippet: Cytokine detection in the tear fluid of KC and non-KC patients, as well as in conditioned medium, was conducted using array-based technology Proteome Profiler Human XL Cytokine Array Kit (Cat no ARY022B, R and D Systems, Minneapolis, USA) following the manufacturer’s instructions.

Techniques: Zymography, Staining

Journal: Biomaterials science

Article Title: Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models.

doi: 10.1039/d4bm01569j

Figure Lengend Snippet: Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

Article Snippet: Cytokine profiling of the EV groups was performed using the dot blot-based Proteome Profiler Human XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions.

Techniques: